Local electrostatic interactions determine the diameter of fusion pores
نویسندگان
چکیده
منابع مشابه
Regulation of Exocytosis and Fusion Pores by Synaptotagmin-Effector Interactions
Synaptotagmin (syt) serves as a Ca(2+) sensor in the release of neurotransmitters and hormones. This function depends on the ability of syt to interact with other molecules. Syt binds to phosphatidylserine (PS)-containing lipid bilayers as well as to soluble N-ethylmaleimide sensitive factor receptors (SNAREs) and promotes SNARE assembly. All these interactions are regulated by Ca(2+), but thei...
متن کاملFusion proteins and fusion pores
A meeting was recently held at the Juan March Foundation in Madrid (22–24 May, 2000) on the topic ‘Regulated exocytosis and the vesicle cycle’. This small discussion meeting brought together speakers using molecular, electrophysiological or a combination of approaches in the study of exocytosis and vesicle retrieval. It was clear from the presentations and discussions that there is now reasonab...
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The fusion pore is an aqueous channel that is formed upon the fusion of the vesicle membrane with the plasma membrane. Once the pore is open, it may close again (transient fusion) or widen completely (full fusion) to permit vesicle cargo discharge. While repetitive transient fusion pore openings of the vesicle with the plasma membrane have been observed in the absence of stimulation, their freq...
متن کاملLocal and macroscopic electrostatic interactions in single α-helices.
The noncovalent forces that stabilize protein structures are not fully understood. One way to address this is to study equilibria between unfolded states and α-helices in peptides. Electrostatic forces-which include interactions between side chains, the backbone and side chains, and side chains and the helix macrodipole-are believed to contribute to these equilibria. Here we probe these interac...
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Membrane fusion at endomembranes requires cross-talk between Rab GTPases and tethers to drive SNARE-mediated lipid bilayer mixing. Several tethers have multiple Rab-binding sites with largely untested function. Here we dissected the lysosomal HOPS complex as a tethering complex with just two binding sites for the Rab7-like Ypt7 protein to determine their relevance for fusion. Using tethering an...
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ژورنال
عنوان ژورنال: Channels
سال: 2015
ISSN: 1933-6950,1933-6969
DOI: 10.1080/19336950.2015.1007825